Current Acceptable Standards

Additional Information for research involving Viruses or Vectors

Genetic manipulations that have the potential to increase pathogenicity may raise the recommended BSL, and may also require review by the federal Recombinant Advisory Committee (RAC).  Below are the Current Acceptable Standards for the University of Pittsburgh recommended by the IBC:

Type of Virus or VectorRecommended BSLStrain example
Murine Retrovirus, ecotropic1 or 1+MMLV, MSCV
Murine Retrovirus, amphotropic or VSV-G pseudotyped 1+, 2 or 2+MMLV, MSCV
Non-primate Lentiviruses2 or 2+FIV, BIV, EIAV
Primate Lentiviruses1, 2, or 2+HIV, SIV, SHIV
Adenoviruses2 or 2+Adv, all types
Adeno-Associated Virus (AAV)1, 2, or 2+AAV
Gene editing technologies 1, 2, or 2+CRISPR/Cas9, TALEN, ZFN
Zika Virus Information 2ZIKV

*Note: Some HIV-1 based lentiviral vector systems may be safely used under lower containment (BSL-2 or ABSL-1)

For additional information contact the IBC Office.

Page updated 15 May 2019; updated table, Gene editing, and Primate Lentiviral guidances