Current Acceptable Standards

Additional Information for research involving Viruses or Vectors

Genetic manipulations that have the potential to increase pathogenicity may raise the recommended BSL, and may also require review by the federal Recombinant Advisory Committee (RAC).  Below are the Current Acceptable Standards for the University of Pittsburgh recommended by the IBC:

Type of Virus or VectorRecommended BSLStrain example
Murine retrovirus, Ecotropic1 or 1+MMLV, MMSV
Murine retrovirus, Amphotropic or VSV-G pseudotyped 1+ or 2MMLV, MMSV
Non-primate Lentiviruses2FIV, BIV, EIAV
Primate Lentiviruses1-3; depending on criteriaHIV, SIV, SHIV
Adenoviruses2Adv, all types
Adeno-Associated Virus (AAV)1 or 2AAV-5, AAV-8
CRISPR/Cas-9 gene  1 or 2Gene Drive technology
Zika Virus Information 2Flavivirus family

*Note: Some HIV-1 based lentiviral vector systems may be safely used under lower containment (BSL-2 or ABSL-1)

For additional information contact the IBC Office.

Page updated 27 August 2018; updated LVV Usage Guidance (Primate)